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KMID : 0861020220370060001
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Korea Journal of Herbology
2022 Volume.37 No. 6 p.1 ~ p.8
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Study on Chondro-protective and Anti-inflammatory Effects of Caraganae Sinicae Flos Extract
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Park Dong-Jun
Lee Hong-Gu
Park Hyoung-Kook
Jin Mu-Hyun
Cho Ho-Song
Ko Chung-Ho
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Abstract
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Objectives : This research aimed to investigate chondro-protective and anti-inflammatory effects of Caraganae
Sinicae Flos 50% ethanol extract and its compound, tilianin.
Methods : Caraganae Sinicae Flos was extracted with 50% ethanol. Tilianin in Caraganae Sinicae Flos 50% ethanol
extract was quantified by HPLC analysis method. To investigate chondro-protective effects of Caraganae Sinicae Flos
50% ethanol extract, ATDC5 chondrogenic cells were co-treated with Caraganae Sinicae Flos 50% ethanol extract (or
tilianin) and tumor necrosis factor-α (TNFα) for 24 hours. After treatement for 24 hours, media supernatant was used for quantifying protein level of matrix metalloproteinase-3 (MMP3) by ELISA and harvested cells were used for
analyzing mRNA expression level of matrix metalloproteinase-13 (MMP13) by reverse transcription PCR. To identify
anti-inflammatory effects of Caraganae Sinicae Flos 50% ethanol extract, RAW 264.7 macrophage cells were co- treated with Caraganae Sinicae Flos 50% ethanol extract (or tilianin) and lipopolysaccharide (LPS) for 24 hours.
media was used for quantifying the level of prostaglandin E2 (PGE2), interleukin-6 (IL6) by ELISA and nitric oxide
by Griess reagent asssay.
Results : Caraganae Sinicae Flos 50% ethanol extract and tilianin attenuated protein level of MMP3 and mRNA
expression level of MMP13 in TNFα-activated ATDC5 cells. Caraganae Sinicae Flos 50% ethanol extract inhibited
the level of PGE2, IL6 and NO in LPS-activated RAW 264.7 cells in dose dependent manner, though tilianin inhibited
PGE2 only.
Conclusions : These results presented that Caraganae Sinicae Flos 50% ethanol extract could be used as natural
medicines for osteoarthritis. 1)
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KEYWORD
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Caraganae Sinicae Flos, Tilianin, Chondro-protective effects, Anti-inflammatory Activities
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